facultyfaculty.htmlshapeimage_1_link_0
educationeducation.htmlshapeimage_2_link_0
wassarmanwassarman.htmlshapeimage_3_link_0
homehome.htmlshapeimage_4_link_0
seminarsseminars.htmlshapeimage_5_link_0
facilitiesfacilities.htmlshapeimage_6_link_0
linkslinks.htmlshapeimage_7_link_0
peoplewassarmanlab.htmlshapeimage_8_link_0
http://www.mountsinai.org/Education/School%20of%20Medicine
home.html
linkswassarmanlink.htmlshapeimage_12_link_0
http://gallery.me.com/drbsinai#100039&bgcolor=black&view=mosaic&sel=0
 

Wassarman Lab - Publications

3wassarman.html

Publications since:


2009

Wassarman, P.M. and Litscher, E.S.: The multifunctional zona pellucida and mammalian fertilization. Journal Reproductive Immunology 83: 45-49.

Litscher, E.S., Williams, Z., and Wassarman, P.M.: Zona pellucida glycoprotein ZP3 and fertilization in mammals.  Molecular Reproduction and Development 76: 933-941.

Wassarman, P.M.:  Mammalian fertilization:  The strange case of sperm protein 56.  BioEssays 31: 153-158.


2008

Darie, C.C., Litscher, E.S., and Wassarman, P.M.: Structure, processing, and polymerization of trout egg vitelline envelope proteins. In, Mass Spectrometry in the Life Sciences (Popescu, C., Zamfir, A., and Dinca, N., eds.), pp. 23-36, Springer-Verlag, Heidelberg.

Wassarman, P.M.: Fertilization - Welcome to the fold. Nature 456: 586-587.

Litscher, E.S., Janssen, W.G., Darie, C.C., and Wassarman, P.M.: Purified mouse egg zona pellucida glycoproteins polymerize into homomeric fibrils under non-denaturing conditions. Journal Cellular Physiology 214: 153-157 (2008).

Wassarman, P.M. and Litscher, E.S.: Mammalian fertilization is dependent on multiple membrane fusion events.  In, Cell Fusion: Methods and Protocols 475: 99-113 (Chen, E.H., ed.), Methods in Molecular Biology, Humana Press, Totawa, NJ (2008).

Darie, C.C., Janssen, W.G., Litscher, E.S., and Wassarman, P.M.: Purified trout egg vitelline envelope proteins VEb and VEg polymerize into homomeric fibrils from dimers in vitro. Biochimica Biophysica Acta (Proteins and Proteomics) 1784: 385-392 (2008).

Wassarman, P.M. and Vacquier, V.D. (eds.): Fertilization. International Journal Developmental Biology (Special issue) 52: 409-796 (2008).

Wassarman, P.M. and Litscher, E.S.: Mammalian fertilization: The egg's multifunctional zona pellucida. International Journal Developmental Biology 52: 665-676 (2008).

Wassarman, P.M.:  Zona pellucida glycoproteins. Journal Biological Chemistry 283: 24285-24289 (2008).


2007

Jovine, L., Qi, H., Williams, Z., Litscher, E.S., and Wassarman, P.M.:  Features that affect secretion and assembly of zona pellucida glycoproteins during mammalian oogenesis.  In, Gamete Biology (S. Gupta K. Koyama, and J.F. Murray, eds.), Nottingham Univ. Press, pp. 187-202 (2007).

Kohn, M.J., Kaneko, K.J., Yagi, R., Litscher, E.S., Wassarman, P.M., DePamphilis, M.L.:  Dickkopf-like 1- a protein unique to mammals that is associated both with formation of trophoblast stem cells and with spermatogenesis. In, Y Chromosome and Male Germ Cell Biology in Health and Diseases (Lau, Y.-F.C. and Chan, W.Y., eds.), World Scientific Publishers, Hackensack, NJ, pp. 185-199 (2007).


2006

Williams, Z., Litscher, E.S., Jovine, L., and Wassarman, P.M.: Polypeptide encoded by mouse ZP3 exon-7 is necessary and sufficient for sperm binding in vitro.  Journal Cellular Physiology 207: 30-39 (2006).

Jovine, L., Janssen, W.G., Litscher, E.S., and Wassarman, P.M.: The PLAC1-homology region of the ZP domain is sufficient for protein polymerization.  BMC Biochemistry 7: 11 (9 pp.) (2006).


2005

Jovine, L., Darie, C.C., Litscher, E.S., and Wassarman, P.M.: Zona pellucida domain proteins. Annual Review Biochemistry 74: 83-114 (2005).

Wassarman, P.M., Jovine, L., Qi, H., Williams, Z., Darie, C., and Litscher, E.S.: Recent aspects of mammalian fertilization research.  Molecular Cellular Endocrinology  234: 95-103 (2005).

Wassarman, P.M.:  Contribution of mouse egg zona pellucida glycoproteins to gamete recognition during fertilization.  Journal Cellular Physiology 204: 388-391 (2005).

Darie, C.C., Biniossek, M.L., Thumfart, J.O., Gawinowicz, M.A., Milgrom, Y., Jovine, L., Litscher, E.S., and Wassarman, P.M.:  Mass spectrometric evidence that proteolytic processing of rainbow trout egg vitelline envelope proteins takes place on the egg.  Journal Biological Chemistry 280: 37585-37598 (2005).


2004

Jovine, L., Qi, H., Williams, Z., Litscher, E.S., and Wassarman, P.M.:  A duplicated motif controls assembly of zona pellucida domain proteins.  Proceedings National Academy Sciences, USA  101: 5922-5927 (2004).

Darie, C.C., Biniossek, M.L., Jovine, L., Litscher, E.S., and Wassarman, P.M.:  Structural characterization of fish egg vitelline envelope proteins by mass spectrometry.  Biochemistry 43: 7459-7478 (2004).


2003

Wassarman, P.M. and Keller, G. (eds.):  Differentiation of embryonic stem cells.  Methods in Enzymology, Vol. 365, Academic Press, San Diego, CA, 535 pp. (2003).


2002

Qi, H., Williams, Z.,  and Wassarman, P.M.:  Secretion and assembly of zona pellucida glycoproteins by growing mouse oocytes microinjected with epitope-tagged cDNAs for mZP2 and mZP3.  Molecular Biology Cell 13:  530-541 (2002).

Wassarman, P.M.:  Channels of communication in the ovary.  Nature Cell Biology 4 (S1): S7-S9 (2002).

Jovine, L., Qi, H., Williams, Z., Litscher, E., and Wassarman, P.M.:  The ZP domain is a conserved module for protein polymerization. Nature Cell Biology 4:  457-461 (2002). 

Jovine, L., Park, J., and Wassarman, P.M.:  Sequence similarity between stereocilin and otoancorin points to a unified mechanism for mechanotransduction of sound in the inner ear.  BMC Cell Biology 3:  28 (2002).


2001

Williams, Z. and Wassarman, P.M.:  Secretion of mouse ZP3, the sperm receptor, requires cleavage of its polypeptide at a consensus furin cleavage site.  Biochemistry 40:  929-937 (2001).

Wassarman, P.M., Jovine, L., and Litscher, E.S.:  A profile of fertilization in mammals.  Nature Cell Biology 3: E59-E64 (2001).

Cohen, N. and Wassarman, P.M.:  Association of egg zona pellucida glycoprotein mZP3 with sperm protein sp56 during fertilization in mice. International Journal Developmental Biology 45: 569-576 (2001).


1999

Wassarman, P.M.:  Mammalian fertilization:  Molecular aspects of gamete adhesion, exocytosis, and fusion.  Cell 96: 175-183 (1999).

Wassarman, P.M. and Wolffe, A.P. (eds.), Chromatin, Methods in Enzymology, Vol. 304, Academic Press, San Diego, CA, 815 pp.  (1999).

Qi, H. and Wassarman, P.M.:  Secretion of zona pellucida glycoprotein mZP2 by growing oocytes from mZP3+/+ and mZP3-/- mice.  Developmental Genetics 25: 95-102 (1999).

Litscher, E.S., Qi, H., and Wassarman, P.M.:  Mouse zona pellucida glycoproteins mZP2 and mZP3 undergo carboxy-terminal proteolytic processing in growing oocytes.  Biochemistry 38:  12280-12287 (1999).

Litscher, E.S., Liu, C., Echelard, Y., and Wassarman, P.M.:  Zona pellucida glycoprotein mZP3 produced in milk of transgenic mice is active as a sperm receptor, but can be lethal to newborns.  Transgenic Research 8:  361-369 (1999).


1998

Chen, J., Litscher, E.S., and Wassarman, P.M.:  Inactivation of the mouse sperm receptor, mZP3, by site-directed mutagenesis of individual serine residues located at the combining site for sperm.  Proceedings National Academy of Sciences, USA 95:  6193-6197 (1998).


1997

Liu, C., Litscher, E.S, and Wassarman, P.M.:  Zona pellucida glycoprotein mZP3 bioactivity is not dependent on the extent of glycosylation of its polypeptide or on sulfation and sialylation of its oligosaccharides.  Journal Cell Science 110:  745-752 (1997).

Wassarman, P.M., Qi, H., and Litscher, E.S.:  Mutant female mice carrying a single mZP3 allele produce eggs with a thin zona pellucida, but reproduce normally. Proceedings Royal Society, Biological Sciences 264:  323-328 (1997).


1996

Litscher, E.S. and Wassarman, P.M.:  Characterization of a mouse ZP3-derived glycopeptide, gp55, that exhibits sperm receptor and acrosome reaction-inducing activity in vitro.  Biochemistry  35:  3980-3986 (1996).

Liu, C., Litscher, E.S., Mortillo, S., Sakai, Y., Kinloch, R.A., Stewart, C.L., and Wassarman, P.M.:  Targeted disruption of  the mZP3 gene results in production of eggs lacking a zona pellucida and infertility in female mice.  Proceedings National Academy of Sciences, USA 93:  5431-5436 (1996).

Wassarman, P.M., Liu, C., and Litscher, E.S.:  Constructing the mouse egg zona pellucida:  some new pieces of an old puzzle.  Journal Cell Science 109:  2001-2004 (1996).

Litscher, E.S. and Wassarman, P.M.:  Recombinant hamster sperm receptors that exhibit species-specific binding to sperm.  Zygote  4:  229-236 (1996).


1995

Kinloch, R.A., Sakai, Y., and Wassarman, P.M.:   Mapping the mouse ZP3 combining site for sperm by exon swapping and site-directed mutagenesis.  Proceedings National Academy of Sciences, USA 92:  263-267  (1995).

Litscher, E.S, Juntunen, K., Seppo, A., Niemalä, R., Penttilä, L., Renkonen, O., and Wassarman, P.M.:   Oligosaccharide constructs with defined structures that inhibit binding of mouse sperm to unfertilized eggs in vitro.   Biochemistry  34:  4662-4669 (1995).

Liu, C., Litscher, E.S., and Wassarman, P.M.:  Transgenic mice that reproduce normally despite reduced numbers of functional sperm receptors on their eggs.  Molecular Biology Cell 6:  577-585 (1995).

Wassarman, P.M. and Litscher, E.S.:  Sperm-egg recognition mechanisms in mammals.  In,  Current Topics in Developmental Biology, Vol. 30 (R.A. Pedersen and G. Schatten, eds.), Academic Press, Orlando, FL,  pp. 1-19 (1995).

Wassarman, P.M.:  Towards molecular mechanisms for gamete adhesion and fusion during mammalian fertilization.  Current Opinion Cell Biology 7: 658-664  (1995).


1994

Wassarman, P.M. and Albertini, D.F.:  Cellular and molecular biology of the mammalian ovum.  In,  The Physiology of Reproduction  (E. Knobil and J.D. Neill, eds.), Vol. 1, 2nd ed., Raven Press, NY, pp. 79-122  (1994).

Cheng, A., Le, T., Palacios, M., Bookbinder, L.H., Wassarman, P.M., F. Suzuki, and Bleil, J.D.:  Sperm-egg recognition in the mouse:  Characterization of sp56, a sperm protein having specific affinity for ZP3.  Journal Cell Biology  125:  867-878 (1994).


1993

Wassarman, P.M.:  Mammalian fertilization: Sperm receptor genes and glycoproteins.  Advances Developmental Biochemistry  2:  155-199 (1993).

Wassarman, P.M.:  Mammalian eggs, sperm, and fertilization:  Dissimilar cells with a common goal.  Seminars Developmental Biology 4:  189-197 (1993).

Wassarman, P.M. and DePamphilis, M.L. (eds.): Guide to Techniques in Mouse Development, Methods in Enzymology, Vol. 225, Academic Press, Orlando, FL, 1021 pp. (1993).

Lira, S.A., Schickler, M., and Wassarman, P.M.:  Cis-acting DNA elements involved in oocyte-specific expression of mouse sperm receptor gene mZP3 are located close to the gene's transcription start-site.  Molecular Reproduction Development 36: 494-499  (1993).

Richards, W.G., Carroll, P.M., Kinloch, R.A., Wassarman, P.M., and Strickland, S.:  Creating maternal effect mutations in transgenic mice:  Antisense inhibition of a specific oocyte gene product.  Developmental Biology  160:  543-553  (1993).


1992

Schickler, M., Lira, S.A., Kinloch, R.A., and Wassarman, P.M.:  A mouse oocyte-specific protein that binds to a region of mZP3 promoter involved in regulating oocyte-specific expression of the mZP3 gene.  Molecular Cellular Biology 12: 120-127 (1992).

Kinloch, R.A., Mortillo, S., and Wassarman, P.M.:  Transgenic mouse eggs with functional hamster sperm receptors in their zona pellucida.  Development 115:  937-946 (1992).

Rosiere, T.K. and Wassarman, P.M.:  Identification of a region of mouse zona pellucida glycoprotein mZP3 that possesses sperm receptor activity.  Developmental Biology 154:  309-317 (1992).


1991

Mortillo, S. and Wassarman, P.M.:  Differential binding of gold-labeled zona pellucida glycoproteins mZP2 and mZP3 to mouse sperm membrane compartments.  Development 113:  141-149 (1991).

Kinloch, R.A., Mortillo, S., Stewart, C.L., and Wassarman, P.M.:  Embryonal carcinoma cells transfected with ZP3 genes differentially glycosylate similar polypeptides and secrete active mouse sperm receptor.  Journal Cell Biology  115:  655-664 (1991).


1990

Wassarman, P.M.:  Profile of a mammalian sperm receptor.  Development  108:  1-19 (1990).

Moller, C.C., Bleil, J.D., Kinloch, R.A., and Wassarman, P.M.:  Structural and functional relationships between mouse and hamster zona pellucida glycoproteins.  Developmental Biology 137: 276-286 (1990).

Bleil, J.D. and Wassarman, P.M.:  Identification of a ZP3-binding protein on acrosome-intact mouse sperm by photoaffinity crosslinking.  Proceedings National Academy of Sciences, USA  87: 5563-5567 (1990).

Lira, S.A., Kinloch, R.A., Mortillo, S., and Wassarman, P.M.:  An upstream  region of the mouse ZP3 gene directs expression of firefly luciferase specifically  to growing oocytes in transgenic mice.  Proceedings National Academy of Sciences, USA  87: 7215-7219 (1990).

Kinloch, R.A., Ruiz-Seiler, B., and Wassarman, P.M.:  Genomic organization  and polypeptide primary structure of zona pellucida glycoprotein hZP3, the, hamster sperm receptor.  Developmental Biology  142: 414-421 (1990).


1989

Wassarman, P.M.:  Role of carbohydrates in receptor-mediated fertilization in mammals.  In, Carbohydrate Recognition in Cellular Function (Bock, G. and Harnett, S., eds.), Ciba Foundation Symposia (No. 145), John Wiley and Sons, Chichester, pp. 135-155 (1989).

Moller, C.C. and Wassarman, P.M.:  Characterization of a proteinase that cleaves zona pellucida glycoprotein ZP2 following activation of mouse eggs.  Developmental Biology  132: 103-112 (1989).

Wassarman, P.M. and Kornberg, R.D. (eds.), Nucleosomes, Methods in Enzymology, Vol. 170, Academic Press, Orlando, FL, 683 pp. (1989).

Vazquez, M.H., Phillips, D.M., and Wassarman, P.M.:  Interaction of mouse sperm  with purified sperm  receptors covalently-linked to silica beads. Journal Cell Science  92:  713-722 (1989).

Roller, R.J., Kinloch, R.A., Hiraoka, B.Y., Li, S.S.-L., and Wassarman, P.M.: Gene expression during mammalian oogenesis and early embryogenesis:  Quantification of three messenger-RNAs abundant in fully-grown mouse  oocytes.  Development  106:  251-261 (1989).

Brown, J., Cebra-Thomas, J.A., Bleil, J.D., Wassarman, P.M., and Silver, L.M.:  A premature acrosome reaction is programmed by mouse t haplotype during  sperm differentiation and could play a role in transmission ratio distortion.  Development  106:  769-773 (1989).


1988

Wassarman, P.M.:  The mammalian ovum.  In, The Physiology of Reproduction (E. Knobil and J.D. Neill, eds.), Vol. 1, Raven Press, NY, pp. 69-102 (1988).

Wassarman, P.M.:  Zona pellucida glycoproteins.  Annual Review Biochemistry 57:  415-442 (1988).

Wassarman, P.M.:  Fertilization in mammals.  Scientific American 255:  78-84 (December, 1988).

Bleil, J.D., Greve, J.M., and Wassarman, P.M.:  Identification of a secondary sperm receptor in the mouse egg zona pellucida:  Role in maintenance of binding of acrosome-reacted sperm to eggs.  Developmental Biology 128:  376-385 (1988).

Kinloch, R.A., Roller, R.J., Fimiani, C.M., Wassarman, D.A., and Wassarman, P.M.:  Primary structure of the mouse sperm receptor's polypeptide chain determined by genomic cloning.  Proceedings National Academy of Sciences, USA 85:  6409-6413 (1988).

Bleil, J.D. and Wassarman, P.M.:  Galactose at the nonreducing terminus of O-linked oligosaccharides of mouse egg zona pellucida glycoprotein ZP3 is essential for the glycoprotein's sperm receptor activity.  Proceedings National Academy of Sciences, USA  85:  6778-6782 (1988).


1987

Wassarman, P.M.:  The biology and chemistry of fertilization.  Science  235:  553-560 (1987).

Wassarman, P.M.:  Early events in mammalian fertilization.  Annual Review Cell Biology  3:  109-142 (1987).

Wassarman, P.M.:  The zona pellucida:  A coat of many colors.  BioEssays  6:  161 (1987) [cover image].

DePamphilis, M.L. and Wassarman, P.M.:  Regulation of chromosomal replication and transcription during early mammalian development.  BioEssays  6:  265 (1987).


1986

Perona, R.M. and Wassarman, P.M.:  Mouse blastocysts hatch in vitro by using a trypsin-like proteinase associated with cells of mural trophectoderm.  Developmental Biology 114:  42-52 (1986).

Bleil, J.D. and Wassarman, P.M.:  Autoradiographic visualization of the mouse egg's sperm receptor bound to sperm.  Journal Cell Biology 102:  1363-1371 (1986).


1985

Greve, J.M. and Wassarman, P.M.:  Mouse egg extracellular coat is a matrix of interconnected filaments possessing a structural repeat.  Journal Molecular Biology 181:  253-264 (1985).

Florman, H.M. and Wassarman, P.M.:  O-Linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity.  Cell 41:  313-324 (1985).

Wassarman, P.M., Bleil, J.D., Florman, H.M., Greve, J.M., Roller, R.J., Salzmann, G.S., and Samuels, F.G.:  The mouse egg's sperm receptor:  What is it and how does it work?  In, Molecular Biology of Development, Cold Spring Harbor Symposium Quantitative Biology  50:  11-18 (1985).