ES cell culture

References, reagents and conditions for ES cell culture, electroporation and expansion.

 

PCR

Buffer, reaction mix and sample prep for cells and tissue.

PCR screening of ES cell clones

Reagents and procedure for identification of targeted clones

 

Southern blot

Processing gel, transfer, probing and washing, and exposure to film.

 

Southern blot screening of ES cell clones

Reference to protocol adapted to 96 well plate format.

X-gal staining of cell lines and embryos

Solutions, volumes and fixation times for cells and embryos.

© 2008 Mount Sinai Medical Center